V Congreso Latinoamericano de Ingeniería Biomédica (CLAIB2011)

Estudio Preliminar de Liberación Controlada de una Proteína Modelo, Empleando como Soporte el Complejo Interpolimérico Poli(cloruro de acriloiloxietil–trimetilamonio – co – metacrilato de 2-hidroxietilo)/alginato de sodio
Castro Fernández Ariadne, Valdés Lizama Oscar

##manager.scheduler.building##: Palacio de Convenciones de La Habana
##manager.scheduler.room##: Sala 12
Fecha: 2011-05-20 09:00  – 10:30
Última modificación: 2011-04-14 12:21

Resumen


Novel interpolymeric microcapsules based on a polylectrolyte complexation between sodium alginate (AlgNa) and poly(acryloxyethyl-trimethylammonium chloride-co-2-hydroxyethyl methacrylate) (poly(Q9/HEMA)) are used for a preliminary study of controlled release of a protein, in this case Bovine Serum Albumin protein (BSA). In the procedure of microcapsule formation alginate was first pumped into a CaCl2 solution and then transferred into a poly(Q9/HEMA) solution. The poly(Q9/HEMA) was prepared by free radical polymerization in aqueous solution using potassium persulfate as initiator. The copolymer and complex formation and microcapsules morphologies were studied by RMN-1H and RMN-13C spectroscopies and AFM and SEM microscopies, respectively. The ability to release the active substance was estudied at pH 1,5 and 6,8 in Gastric and Intestinal Simulated Fluids respectively. The release of BSA is prevented at acidic pH, while is complete in a few minutes when pH is raised up to 6.8.